WEKO3
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Identification and functional analysis of a novel mitochondria-localized 2-Cys peroxiredoxin, BbTPx-2, from Babesia bovis
名前 / ファイル | ライセンス | アクション |
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BbovTPx2.pdf (432.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2017-09-07 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Identification and functional analysis of a novel mitochondria-localized 2-Cys peroxiredoxin, BbTPx-2, from Babesia bovis | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Antioxidant activity | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Babasia bovis | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Mitochondria | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Peroxiredoxin | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Masatani, Tatsunori
× Masatani, Tatsunori
WEKO
348
× Asada, Masahito× Hakimi, Hassan× Hayashi, Kei× Yamagishi, Junya
WEKO
330
× 河津, 信一郎× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Cysteine-based peroxidases, known as peroxiredoxins (Prx) or thioredoxin peroxidases (TPx), are important antioxidant enzymes that prevent oxidative damage caused by reactive oxygen species (ROS). In this study, we identified a novel mitochondrial 2-Cys Prx, BbTPx-2, from a bovine Babesia parasite, B. bovis. BbTPx-2 complementary DNA (cDNA) encodes a polypeptide of 254 amino acid residues. This protein has a mitochondrial targeting peptide at the N-terminus and two conserved cysteine residues of the typical 2-Cys Prx. By using a thiol mixed-function oxidation assay, the antioxidant activity of recombinant BbTPx-2 was revealed, and its antioxidant activity was comparable to that of a cytosolic 2-Cys Prx from B. bovis, BbTPx-1. Notably, we confirmed that BbTPx-2 was expressed in the mitochondrion of B. bovis merozoites. Taken together, the results suggest that the mitochondrial BbTPx-2 is an antioxidative enzyme for scavenging ROS in B. bovis. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Parasitology Research 巻 115, 号 8, p. 3139-3145, 発行日 2016-08 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | Springer | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 09320113 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10682117 | |||||||||||||
DOI | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | info:doi/10.1007/s00436-016-5071-9 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | The final publication is available at Springer via http://doi.org/10.1007/s00436-016-5071-9. | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | AM | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
日本十進分類法 | ||||||||||||||
主題Scheme | NDC | |||||||||||||
主題 | 491 |