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Incidence of apoptotic cells after vitrification in canine ovarian tissues
https://obihiro.repo.nii.ac.jp/records/1180
https://obihiro.repo.nii.ac.jp/records/118054ab8bc0-e845-4ef1-8f00-22c8d9f0156d
名前 / ファイル | ライセンス | アクション |
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jmor.33.69.pdf (979.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Incidence of apoptotic cells after vitrification in canine ovarian tissues | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cryopreservation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Dog | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Follicle | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Ovary | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hariya, Madoka
× Hariya, Madoka× Suzuki, Hiroshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved ovarian tissues were fixed and subsequently stained with hematoxylin-eosin, proliferation cell nuclear antigen (PCNA) antibody, or active caspase-3 (AC-3) antibody, and evaluated by the TUNEL assay. There were no differences in the numbers of primordial, primary, secondary and antral follicles/mm2 between the fresh and vitrified-warmed ovarian tissues. Percentages of PCNA and AC-3 antibody positive cells were similar regardless of the developmental stage of the follicles and experimental group. A few TUNEL positive cells were detected in both experimental groups. These results suggest that vitrification with DAP213 does not induce cryoinjury in ovarian tissues from immature bitches. c 2016 Japan Society for Ova Research. | |||||
言語 | en | |||||
書誌情報 |
en : Journal of Mammalian Ova Research 巻 33, 号 1, p. 69-75, 発行日 2016 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Japan Society for Ova Research | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 13417738 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10548943 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Japan Society for Ova Research | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 491 |