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アイテム
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Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1
名前 / ファイル | ライセンス | アクション |
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serodiagnosis.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||
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公開日 | 2011-07-20 | |||||||||||||||||||||||
タイトル | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
タイトル | Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1 | |||||||||||||||||||||||
言語 | ||||||||||||||||||||||||
言語 | eng | |||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||
主題 | ELISA | |||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||
主題 | Mongolia | |||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||
主題 | sheep | |||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||
主題 | TgMAG1 | |||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||
主題 | Toxoplasma gondii | |||||||||||||||||||||||
資源タイプ | ||||||||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
資源タイプ | journal article | |||||||||||||||||||||||
著者 |
Tumurjav, Buyannemekh
× Tumurjav, Buyannemekh× Terkawi, Mohamad Alaa
WEKO
359
× Zhang, Houshuang× Zhang, Guohong× Jia, Honglin× Goo, Youn-Kyoung× Yamagishi, Junya
WEKO
330
× 西川, 義文
WEKO
176
× 五十嵐, 郁男
WEKO
58
× Sugimoto, Chihiro
WEKO
252
× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||||||||
内容記述 | Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep in 7 provinces of Mongolia were examined for the serodiagnosis of T. gondii infection by the ELISA with rTgMAG1, and the results were compared with those from the commercialized latex agglutination test (LAT). Of 175 serum samples analyzed, 42(24.00%) and 29 (16.57%) samples were positive by the ELISA and LAT, respedtively. Of 29 LAT-positive samples, 27 (93.10%) were positive by the ELISA. These results suggest that rTgMAG1 could be used as a reliable antigen for the detection of T. gondii infection in sheep. | |||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
書誌情報 |
en : Japanese Journal of Veterinary Research 巻 58, 号 2, p. 111-119, 発行日 2010-08 |
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出版者 | ||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||
出版者 | The Graduate School of Veterinary Medicine, Hokkaido University | |||||||||||||||||||||||
ISSN | ||||||||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||||||||
収録物識別子 | 00471917 | |||||||||||||||||||||||
書誌レコードID | ||||||||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||||||||
収録物識別子 | AA0069128X | |||||||||||||||||||||||
DOI | ||||||||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||||||||
関連識別子 | info:doi/10.14943/jjvr.58.2.111 | |||||||||||||||||||||||
フォーマット | ||||||||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||||||||
著者版フラグ | ||||||||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |