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Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis
名前 / ファイル | ライセンス | アクション |
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inoueN070718-1.pdf (810.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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公開日 | 2007-07-18 | |||||||||||||||
タイトル | ||||||||||||||||
言語 | en | |||||||||||||||
タイトル | Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis | |||||||||||||||
言語 | ||||||||||||||||
言語 | eng | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | LAMP | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Trypanosomosis | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Trypanosoma brucei brucei | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | T. b. rhodesiense | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | T. b. gambiense | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | T. congolense | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | T. cruzi | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | T. evansi | |||||||||||||||
資源タイプ | ||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
資源タイプ | journal article | |||||||||||||||
著者 |
Thekisoe, Oriel M. M.
× Thekisoe, Oriel M. M.× Kuboki, Noritaka× Nambota, Andrew× Fujisaki, Kozo× Sugimoto, Chihiro
WEKO
252
× 五十嵐, 郁男
WEKO
58
× Yasuda, Jun× 井上, 昇
WEKO
63
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抄録 | ||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||
内容記述 | In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that lack sufficient resources needed for application of molecular diagnostic techniques. | |||||||||||||||
言語 | en | |||||||||||||||
書誌情報 |
en : Acta Tropica 巻 102, 号 3, p. 182-189, 発行日 2007-06 |
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出版者 | ||||||||||||||||
言語 | en | |||||||||||||||
出版者 | Elsevier | |||||||||||||||
ISSN | ||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||
収録物識別子 | 0001706X | |||||||||||||||
書誌レコードID | ||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||
収録物識別子 | AA00510523 | |||||||||||||||
権利 | ||||||||||||||||
言語 | en | |||||||||||||||
権利情報 | Elsevier | |||||||||||||||
フォーマット | ||||||||||||||||
内容記述タイプ | Other | |||||||||||||||
内容記述 | application/pdf | |||||||||||||||
著者版フラグ | ||||||||||||||||
出版タイプ | VoR | |||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
日本十進分類法 | ||||||||||||||||
主題Scheme | NDC | |||||||||||||||
主題 | 649 |