WEKO3
アイテム
{"_buckets": {"deposit": "37fb8656-e19e-45bc-b344-5988c0baf795"}, "_deposit": {"created_by": 12, "id": "4644", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "4644"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00004644", "sets": ["5"]}, "author_link": ["2485", "61", "147", "261"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "843", "bibliographicPageStart": "836", "bibliographicVolumeNumber": "31", "bibliographic_titles": [{"bibliographic_title": "Journal of Veterinary Diagnostic Investigation", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Circulating cell-free DNA (cfDNA) is extracellular DNA released into the bloodstream by apoptotic or necrotic tumor cells, with cfDNA determination proposed as a noninvasive, sensitive marker for the diagnosis of human cancer. We evaluated cfDNA quantification as a diagnostic and prognostic tool in dogs with various tumors. We quantified plasma cfDNA concentration by absolute real-time PCR of long interspersed nuclear elements in 50 dogs with malignant tumors, 13 dogs with benign tumors or nodules, and 11 healthy controls. Six patients with malignant tumors were followed-up, and plasma cfDNA was quantified throughout disease progression. We found that plasma cfDNA concentrations were significantly elevated in dogs with malignant tumors compared with dogs with benign nodules or healthy controls. The DNA integrity index (the ratio between long and short cfDNA fragments) was significantly lower in dogs with malignant tumors compared to healthy controls. Significantly higher cfDNA levels and a lower DNA integrity index were observed in dogs with lymphoma or leukemia, hemangiosarcoma, and distant metastasis; cfDNA levels correlated well with clinical stage and tended to increase during or before periods of disease progression, suggesting potential efficacy of cfDNA for the detection of distant metastasis and to monitor the clinical stage of neoplasia.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "SAGE Publications", "subitem_publisher_language": "en"}]}, "item_2_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1177/1040638719880245", "subitem_relation_type_select": "DOI"}}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10748543", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "10406387", "subitem_source_identifier_type": "PISSN"}]}, "item_2_subject_21": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "649", "subitem_subject_scheme": "NDC"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "田川道人 mtagawa@obihiro.ac.jp"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Scopus202002-11; 2020/2/20田川先生に依頼(著者版); 2020/2/20 OK,ファイル提供,エンバーゴ; 2020/3/4登録; "}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tagawa, Michihito", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "261", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000254431218", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000254431218"}, {"nameIdentifier": "00749468", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00749468"}]}, {"creatorNames": [{"creatorName": "Shimbo, Genya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2485", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Inokuma, Hisashi", "creatorNameLang": "en"}, {"creatorName": "猪熊, 壽", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Inokuma", "familyNameLang": "en"}, {"familyName": "猪熊", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Hisashi", "givenNameLang": "en"}, {"givenName": "壽", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "61", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070263803", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000070263803"}, {"nameIdentifier": "70263803", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70263803"}, {"nameIdentifier": "read0185156", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0185156"}]}, {"creatorNames": [{"creatorName": "Miyahara, Kazuro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "147", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000005628923", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000005628923"}, {"nameIdentifier": "00133801", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00133801"}, {"nameIdentifier": "read0167495", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0167495"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-03-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "manuscript.pdf", "filesize": [{"value": "2.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2200000.0, "url": {"label": "fulltext", "url": "https://obihiro.repo.nii.ac.jp/record/4644/files/manuscript.pdf"}, "version_id": "d2949260-0caf-4cfc-bb28-c11c59610f6f"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cell-free DNA", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "dogs", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "liquid biopsy", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tumor biomarker", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Quantification of plasma cell-free DNA levels in dogs with various tumors", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Quantification of plasma cell-free DNA levels in dogs with various tumors", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/4644", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2020-03-04"}, "publish_date": "2020-03-04", "publish_status": "0", "recid": "4644", "relation": {}, "relation_version_is_last": true, "title": ["Quantification of plasma cell-free DNA levels in dogs with various tumors"], "weko_shared_id": -1}
Quantification of plasma cell-free DNA levels in dogs with various tumors
https://obihiro.repo.nii.ac.jp/records/4644
https://obihiro.repo.nii.ac.jp/records/464491753998-4ca0-471b-8f62-a8f5dc21593f
名前 / ファイル | ライセンス | アクション |
---|---|---|
fulltext (2.2 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2020-03-04 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Quantification of plasma cell-free DNA levels in dogs with various tumors | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | cell-free DNA | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | dogs | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | liquid biopsy | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | tumor biomarker | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
著者 |
Tagawa, Michihito
× Tagawa, Michihito× Shimbo, Genya× 猪熊, 壽
WEKO
61
× Miyahara, Kazuro
WEKO
147
|
|||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Circulating cell-free DNA (cfDNA) is extracellular DNA released into the bloodstream by apoptotic or necrotic tumor cells, with cfDNA determination proposed as a noninvasive, sensitive marker for the diagnosis of human cancer. We evaluated cfDNA quantification as a diagnostic and prognostic tool in dogs with various tumors. We quantified plasma cfDNA concentration by absolute real-time PCR of long interspersed nuclear elements in 50 dogs with malignant tumors, 13 dogs with benign tumors or nodules, and 11 healthy controls. Six patients with malignant tumors were followed-up, and plasma cfDNA was quantified throughout disease progression. We found that plasma cfDNA concentrations were significantly elevated in dogs with malignant tumors compared with dogs with benign nodules or healthy controls. The DNA integrity index (the ratio between long and short cfDNA fragments) was significantly lower in dogs with malignant tumors compared to healthy controls. Significantly higher cfDNA levels and a lower DNA integrity index were observed in dogs with lymphoma or leukemia, hemangiosarcoma, and distant metastasis; cfDNA levels correlated well with clinical stage and tended to increase during or before periods of disease progression, suggesting potential efficacy of cfDNA for the detection of distant metastasis and to monitor the clinical stage of neoplasia. | |||||||||||
言語 | en | |||||||||||
書誌情報 |
en : Journal of Veterinary Diagnostic Investigation 巻 31, 号 6, p. 836-843, 発行日 2019-11 |
|||||||||||
出版者 | ||||||||||||
言語 | en | |||||||||||
出版者 | SAGE Publications | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 10406387 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA10748543 | |||||||||||
DOI | ||||||||||||
関連タイプ | isVersionOf | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | info:doi/10.1177/1040638719880245 | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | AM | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
日本十進分類法 | ||||||||||||
主題Scheme | NDC | |||||||||||
主題 | 649 |