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Determination of plasma bone-specific alkaline phosphatase isoenzyme activity in Holstein calves using a commercial agarose gel electrophoresis kit
https://obihiro.repo.nii.ac.jp/records/4633
https://obihiro.repo.nii.ac.jp/records/4633a31faecf-1165-43de-8f09-95fdf5b05053
名前 / ファイル | ライセンス | アクション |
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fulltext (930.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2020-02-19 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Determination of plasma bone-specific alkaline phosphatase isoenzyme activity in Holstein calves using a commercial agarose gel electrophoresis kit | |||||||
言語 | ||||||||
言語 | eng | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Agarose gel electrophoresis (AGE) | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Bone-specific alkaline phosphatase isoenzyme | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Calf | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Onomi, R.
× Onomi, R.× Hatate, K.× Yamagishi, Norio
WEKO
300
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | We measured the bone-specific alkaline phosphatase (ALP) isoenzyme activity in 67 plasma samples from 14 newborn Holstein calves using both a conventional method (featuring heat inactivation) and a commercial agarose gel electrophoresis (AGE) kit; the relevant isoenzymes were termed bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3). We explored whether the AGE kit afforded reliable data when used to analyze samples from Holstein calves. The blood was collected from the jugular vein of each calf immediately prior to the first colostrum feeding (pre-feeding), 20 and 40 h after pre-feeding, and on days 4 and 7; whereas three samples (from three calves) were not obtained. The total plasma ALP activity varied widely, exceeding the ranges of reference values. On electrophoresis, 52 of 67 plasma samples (77.6 %) clearly contained both ALP isoenzyme 2 and ALP3, as did control human serum. The total ALP activity of the 52 samples ranged from 166–1989 U/L (median: 1013 U/L), whereas the values for the other 15 samples (22.4%) exhibiting abnormal isoenzyme fractionation ranged from 1014–5118 U/L (median: 1780 U/L). In the 52 plasma samples exhibiting clearly separated isoenzymes, ALP3 and BAP activities were strongly positively correlated as revealed by Deming regression (y = 0.93x + 22.6, p<0.0001) and Bland-Altman analysis (ALP3/BAP activities limit of agreement: −5.1%). Thus, the AGE kit yields useful information on newborn calves, and can replace the conventional method when the total plasma ALP activity is less than approximately 1000 U/L. | |||||||
言語 | en | |||||||
書誌情報 |
en : Polish Journal of Veterinary Sciences 巻 22, 号 4, p. 789-792, 発行日 2019 |
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出版者 | ||||||||
言語 | en | |||||||
出版者 | Polish Academy of Sciences | |||||||
ISSN | ||||||||
収録物識別子タイプ | PISSN | |||||||
収録物識別子 | 15051773 | |||||||
書誌レコードID | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AA12262757 | |||||||
DOI | ||||||||
関連タイプ | isIdenticalTo | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | info:doi/http://doi.org/10.24425/pjvs.2019.131411 | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
日本十進分類法 | ||||||||
主題Scheme | NDC | |||||||
主題 | 649 |