WEKO3
アイテム
{"_buckets": {"deposit": "c30bad0f-73d6-4673-aff4-12929282110e"}, "_deposit": {"created_by": 12, "id": "4385", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "4385"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00004385", "sets": ["5"]}, "author_link": ["356", "6282", "329", "357", "360", "5895", "63", "312"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "1935", "bibliographicPageStart": "1927", "bibliographicVolumeNumber": "118", "bibliographic_titles": [{"bibliographic_title": "Parasitology Research", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Trypanosoma (Megatrypanum) theileri is a cosmopolitan, usually non-pathogenic, trypanosome of cattle transmitted by blood-sucking arthropods, mainly tabanid flies. Several T. theileri strains isolated from domestic and wild ruminants via co-culturing with mammalian feeder cells or blood cells have been characterized morphologically and genetically. Here, we cultured a new trypanosome isolate from a Holstein cow in Hokkaido, Japan, and performed morphological and molecular characterization studies. The new isolate (Obihiro strain) was co-cultivated with Madin–Darby bovine kidney (MDBK) cells in GIT medium supplemented with 10% fetal bovine serum. Trypomastigotes and epimastigotes, but not intracellular parasites, were identified in the culture. Analysis of the V7-V8 region of 18S rRNA sequences showed that the Obihiro strain is positioned within the subgenus Megatrypanum. A dendrogram based on whole internal transcribed spacer rDNA sequence showed that the Obihiro strain clustered in the lineage TthII together with the Japanese isolates of T. theileri, Esashi 9, and Esashi 12, and isolates from Zambia and the USA. T. theileri of the KM strain and a T. theileri-like trypanosome isolated from deer (TSD1 strain) clustered in the lineage TthI, separate from the Obihiro strain. Based on a partial cathepsin L-like protein gene analysis, the Obihiro strain clustered with isolates of the TthIIF genotype, which includes T. theileri from Vietnam, Sri Lanka, and Brazil. Our analyses of the T. theileri Obihiro strain provide relevant insights into its genetic diversity in Japanese cattle and corroborate the host specificity of cattle and deer trypanosomes of the subgenus Megatrypanum. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer", "subitem_publisher_language": "en"}]}, "item_2_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1007/s00436-019-06313-x", "subitem_relation_type_select": "DOI"}}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10682117", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09320113", "subitem_source_identifier_type": "PISSN"}]}, "item_2_subject_21": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "491", "subitem_subject_scheme": "NDC"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "菅沼啓輔 k.suganuma@obihiro.ac.jp"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Scopus201906-6; 2019/6/26菅沼先生に依頼(著者版(author)); 2019/7/2 OK,原稿提供(文字を黒塗りする), エンバーゴ ; 2019/7/29登録; エンバーゴ 2020/6/1;\n"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Suganuma, Keisuke", "creatorNameLang": "en"}, {"creatorName": "菅沼, 啓輔", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Suganuma", "familyNameLang": "en"}, {"familyName": "菅沼", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Keisuke", "givenNameLang": "en"}, {"givenName": "啓輔", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "357", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000345306716", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000345306716"}, {"nameIdentifier": "60772184", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60772184"}, {"nameIdentifier": "11922960", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/11922960"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Kondoh, Daisuke", "creatorNameLang": "en"}, {"creatorName": "近藤, 大輔", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Kondoh", "familyNameLang": "en"}, {"familyName": "近藤", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Daisuke", "givenNameLang": "en"}, {"givenName": "大輔", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "329", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002683176", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002683176"}, {"nameIdentifier": "90708364", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90708364"}, {"nameIdentifier": "0000-0002-3015-5156", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0002-3015-5156"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Sivakumar, Thillaiampalam", "creatorNameLang": "en"}], "familyNames": [{"familyName": "Sivakumar", "familyNameLang": "en"}], "givenNames": [{"givenName": "Thillaiampalam", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "356", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000018822968", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000018822968"}]}, {"creatorNames": [{"creatorName": "Mizushima, Daiki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "5895", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Elata, Afra\u0027a Tajelsir Mohamed", "creatorNameLang": "en"}], "familyNames": [{"familyName": "Elata", "familyNameLang": "en"}], "givenNames": [{"givenName": "Afra\u0027a Tajelsir Mohamed", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6282", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Thekisoe, Oriel M. M.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "360", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000021214199", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000021214199"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Yokoyama, Naoaki", "creatorNameLang": "en"}, {"creatorName": "横山, 直明", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Yokoyama", "familyNameLang": "en"}, {"familyName": "横山", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Naoaki", "givenNameLang": "en"}, {"givenName": "直明", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "312", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002627114", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002627114"}, {"nameIdentifier": "80301802", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80301802"}, {"nameIdentifier": "read0074761", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0074761"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Inoue, Noboru", "creatorNameLang": "en"}, {"creatorName": "井上, 昇", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Inoue", "familyNameLang": "en"}, {"familyName": "井上", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Noboru", "givenNameLang": "en"}, {"givenName": "昇", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "63", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002841346", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002841346"}, {"nameIdentifier": "10271751", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=10271751"}, {"nameIdentifier": "read0185948", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0185948"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "manuscript_rev.pdf", "filesize": [{"value": "2.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2500000.0, "url": {"label": "fulltext", "url": "https://obihiro.repo.nii.ac.jp/record/4385/files/manuscript_rev.pdf"}, "version_id": "0e1acba3-4d59-40f1-ae2e-11f4665588a7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Genotyping", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "In vitro culture", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Megatrypanum", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Morphology", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Stercoraria", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Trypanosoma theileri", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/4385", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-07-29"}, "publish_date": "2019-07-29", "publish_status": "0", "recid": "4385", "relation": {}, "relation_version_is_last": true, "title": ["Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle"], "weko_shared_id": -1}
Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle
名前 / ファイル | ライセンス | アクション |
---|---|---|
fulltext (2.5 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2019-07-29 | |||||||||||||||||||||
タイトル | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
タイトル | Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle | |||||||||||||||||||||
言語 | ||||||||||||||||||||||
言語 | eng | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | Genotyping | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | In vitro culture | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | Megatrypanum | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | Morphology | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | Stercoraria | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | Trypanosoma theileri | |||||||||||||||||||||
資源タイプ | ||||||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
資源タイプ | journal article | |||||||||||||||||||||
著者 |
菅沼, 啓輔
× 菅沼, 啓輔
WEKO
357
× 近藤, 大輔
WEKO
329
× Sivakumar, Thillaiampalam× Mizushima, Daiki× Elata, Afra'a Tajelsir Mohamed× Thekisoe, Oriel M. M.× 横山, 直明
WEKO
312
× 井上, 昇
WEKO
63
|
|||||||||||||||||||||
抄録 | ||||||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||||||
内容記述 | Trypanosoma (Megatrypanum) theileri is a cosmopolitan, usually non-pathogenic, trypanosome of cattle transmitted by blood-sucking arthropods, mainly tabanid flies. Several T. theileri strains isolated from domestic and wild ruminants via co-culturing with mammalian feeder cells or blood cells have been characterized morphologically and genetically. Here, we cultured a new trypanosome isolate from a Holstein cow in Hokkaido, Japan, and performed morphological and molecular characterization studies. The new isolate (Obihiro strain) was co-cultivated with Madin–Darby bovine kidney (MDBK) cells in GIT medium supplemented with 10% fetal bovine serum. Trypomastigotes and epimastigotes, but not intracellular parasites, were identified in the culture. Analysis of the V7-V8 region of 18S rRNA sequences showed that the Obihiro strain is positioned within the subgenus Megatrypanum. A dendrogram based on whole internal transcribed spacer rDNA sequence showed that the Obihiro strain clustered in the lineage TthII together with the Japanese isolates of T. theileri, Esashi 9, and Esashi 12, and isolates from Zambia and the USA. T. theileri of the KM strain and a T. theileri-like trypanosome isolated from deer (TSD1 strain) clustered in the lineage TthI, separate from the Obihiro strain. Based on a partial cathepsin L-like protein gene analysis, the Obihiro strain clustered with isolates of the TthIIF genotype, which includes T. theileri from Vietnam, Sri Lanka, and Brazil. Our analyses of the T. theileri Obihiro strain provide relevant insights into its genetic diversity in Japanese cattle and corroborate the host specificity of cattle and deer trypanosomes of the subgenus Megatrypanum. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. | |||||||||||||||||||||
言語 | en | |||||||||||||||||||||
書誌情報 |
en : Parasitology Research 巻 118, 号 6, p. 1927-1935, 発行日 2019-06 |
|||||||||||||||||||||
出版者 | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
出版者 | Springer | |||||||||||||||||||||
ISSN | ||||||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||||||
収録物識別子 | 09320113 | |||||||||||||||||||||
書誌レコードID | ||||||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||||||
収録物識別子 | AA10682117 | |||||||||||||||||||||
DOI | ||||||||||||||||||||||
関連タイプ | isVersionOf | |||||||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||||||
関連識別子 | info:doi/10.1007/s00436-019-06313-x | |||||||||||||||||||||
フォーマット | ||||||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||||||
著者版フラグ | ||||||||||||||||||||||
出版タイプ | AM | |||||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||
日本十進分類法 | ||||||||||||||||||||||
主題Scheme | NDC | |||||||||||||||||||||
主題 | 491 |