WEKO3
アイテム
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Previously, we showed that VgR mRNA is expressed in the ovary at the adult stage of parthenogenetic Haemaphysalis longicornis ticks and its expression increases after blood-feeding. However, intracellular localization of VgR mRNA and protein at each developmental stage of oocytes during oogenesis remains largely unclear. METHODS: mRNA and protein expression profiles of H. longicornis VgR (HlVgR) in the oocytes from the unfed to oviposition periods were analyzed by real-time PCR, in situ hybridization, and immunostaining. To elucidate the timing of the onset of Vg uptake, RNA interference (RNAi)-mediated gene silencing of HlVgR was performed. RESULTS: In situ hybridization revealed that HlVgR mRNA was detected in the cytoplasm of stage I-III oocytes, and weaker positive signals for HlVgR mRNA were found in the cell periphery of stage IV and V oocytes. Likewise, HlVgR protein was detected by immunostaining in the cytoplasm of stage I-III oocytes and in the cell periphery of stage IV and V oocytes. Each developmental stage of the oocytes showed distinct patterns of mRNA and protein expression of HlVgR. Moreover, RNAi of HlVgR caused delayed or arrested development in the oocytes. The ovaries of control ticks showed all developmental stages of oocytes, whereas stage I-III oocytes were found in the ovaries of HlVgR-RNAi ticks at 5 days after engorgement. CONCLUSIONS: These results suggest that active uptake of Vg is required for development from stage III to stage IV during oogenesis. Our data clearly revealed an apparent shift in the intracellular localization of VgR for both mRNA and protein level in oocytes during oogenesis.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "BioMed Central", "subitem_publisher_language": "en"}]}, "item_2_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1186/s13071-019-3469-9", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. \u003chttp://creativecommons.org/lisenses/by/4.0/\u003e", "subitem_rights_language": "en"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "17563305", "subitem_source_identifier_type": "PISSN"}]}, "item_2_subject_21": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "649", "subitem_subject_scheme": "NDC"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "鈴木宏志 hisuzuki@obihiro.ac.jp"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Scopus201906-2; 2019/6/26鈴木先生に依頼(CC BY); 2019/6/26 OK; 2019/7/29登録; \n"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Umemiya-Shirafuji, Rika", "creatorNameLang": "en"}, {"creatorName": "白藤, 梨可", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Umemiya-Shirafuji", "familyNameLang": "en"}, {"familyName": "白藤", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Rika", "givenNameLang": "en"}, {"givenName": "梨可", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "362", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000365044197", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000365044197"}, {"nameIdentifier": "00549909", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00549909"}, {"nameIdentifier": "0000-0001-7641-5526 ", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0001-7641-5526 "}]}, {"creatorNames": [{"creatorName": "Mihara, Ryo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6278", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujisaki, Kozo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "340", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006874417", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006874417"}, {"nameIdentifier": "00292095", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00292095"}]}, {"creatorNames": [{"creatorName": "Suzuki, Hiroshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "255", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000003006849", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000003006849"}, {"nameIdentifier": "60333473", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60333473"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "document.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "fulltext", "url": "https://obihiro.repo.nii.ac.jp/record/4382/files/document.pdf"}, "version_id": "6c34aa3f-b08c-43f5-b4fa-adbfe52ad4d2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Haemaphysalis longicornis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oocyte", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ovary", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tick", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Vitellogenin receptor", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Intracellular localization of vitellogenin receptor mRNA and protein during oogenesis of a parthenogenetic tick, Haemaphysalis longicornis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Intracellular localization of vitellogenin receptor mRNA and protein during oogenesis of a parthenogenetic tick, Haemaphysalis longicornis", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/4382", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-07-29"}, "publish_date": "2019-07-29", "publish_status": "0", "recid": "4382", "relation": {}, "relation_version_is_last": true, "title": ["Intracellular localization of vitellogenin receptor mRNA and protein during oogenesis of a parthenogenetic tick, Haemaphysalis longicornis"], "weko_shared_id": -1}
Intracellular localization of vitellogenin receptor mRNA and protein during oogenesis of a parthenogenetic tick, Haemaphysalis longicornis
https://obihiro.repo.nii.ac.jp/records/4382
https://obihiro.repo.nii.ac.jp/records/43827d0e8b13-040c-41d0-9a45-9361ee632ae6
名前 / ファイル | ライセンス | アクション |
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fulltext (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2019-07-29 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Intracellular localization of vitellogenin receptor mRNA and protein during oogenesis of a parthenogenetic tick, Haemaphysalis longicornis | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Haemaphysalis longicornis | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Oocyte | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Ovary | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Tick | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Vitellogenin receptor | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
白藤, 梨可
× 白藤, 梨可
WEKO
362
× Mihara, Ryo× Fujisaki, Kozo× Suzuki, Hiroshi |
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | BACKGROUND: Vitellogenin (Vg), a key molecule for oocyte development synthesized in the fat body during blood-feeding, is released into the hemolymph and then taken into the oocytes via Vg receptor (VgR) in ticks. Previously, we showed that VgR mRNA is expressed in the ovary at the adult stage of parthenogenetic Haemaphysalis longicornis ticks and its expression increases after blood-feeding. However, intracellular localization of VgR mRNA and protein at each developmental stage of oocytes during oogenesis remains largely unclear. METHODS: mRNA and protein expression profiles of H. longicornis VgR (HlVgR) in the oocytes from the unfed to oviposition periods were analyzed by real-time PCR, in situ hybridization, and immunostaining. To elucidate the timing of the onset of Vg uptake, RNA interference (RNAi)-mediated gene silencing of HlVgR was performed. RESULTS: In situ hybridization revealed that HlVgR mRNA was detected in the cytoplasm of stage I-III oocytes, and weaker positive signals for HlVgR mRNA were found in the cell periphery of stage IV and V oocytes. Likewise, HlVgR protein was detected by immunostaining in the cytoplasm of stage I-III oocytes and in the cell periphery of stage IV and V oocytes. Each developmental stage of the oocytes showed distinct patterns of mRNA and protein expression of HlVgR. Moreover, RNAi of HlVgR caused delayed or arrested development in the oocytes. The ovaries of control ticks showed all developmental stages of oocytes, whereas stage I-III oocytes were found in the ovaries of HlVgR-RNAi ticks at 5 days after engorgement. CONCLUSIONS: These results suggest that active uptake of Vg is required for development from stage III to stage IV during oogenesis. Our data clearly revealed an apparent shift in the intracellular localization of VgR for both mRNA and protein level in oocytes during oogenesis. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Parasites & vectors 巻 12, 号 1, p. 205, 発行日 2019-05 |
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出版者 | ||||||||||
言語 | en | |||||||||
出版者 | BioMed Central | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 17563305 | |||||||||
DOI | ||||||||||
関連タイプ | isIdenticalTo | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | info:doi/10.1186/s13071-019-3469-9 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.0/> | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
日本十進分類法 | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 649 |