WEKO3
アイテム
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60 Hz electric field upregulates cytosolic Ca2+ level in mouse splenocytes stimulated by lectin.
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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公開日 | 2018-08-14 | |||||||||||||||
タイトル | ||||||||||||||||
言語 | en | |||||||||||||||
タイトル | 60 Hz electric field upregulates cytosolic Ca2+ level in mouse splenocytes stimulated by lectin. | |||||||||||||||
言語 | ||||||||||||||||
言語 | eng | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | concanavalin A | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | phytohemagglutinin | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | alternating cur rent | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | extremely lowfrequency | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | current density | |||||||||||||||
資源タイプ | ||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
資源タイプ | journal article | |||||||||||||||
著者 |
原川, 信二
× 原川, 信二
WEKO
33
× 井上, 昇
WEKO
63
× Saito, Atsushi
WEKO
210
× Doge, Fuyuki× Nagasawa, Hideyuki× Suzuki, Naoyoshi× Martin, David E |
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抄録 | ||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||
内容記述 | The effect of a 60 Hz electric field (EF) on alteration of cytosolic free Ca2+ level ([Ca2+]c) was examined in mouse splenocytes stimulated by lectins, namely concanavalin A (ConA) or phytohemagglutinin. In order to understand the role of EF on alterations in [Ca2+]c and to determine whether EF exposure increased cell mortality the splenocytes were cultured under the 60 Hz EFs producing current densities of 6 or 60 microA/cm2 for 30 min or 24 h. Cell mortality was less than 2% in experimental all conditions. [Ca2+]c in the splenocyte was not changed by the 6 microA/cm2 exposure alone, while a lectin-induced [Ca2+]c elevation in the EF exposed cells was significantly higher than that of the sham exposed cells (P <.05: ANOVA, P <.05: paired t-test). Moreover, the enhanced increase of [Ca2+]c in the EF exposed, lectin stimulated cells was only observed in the presence of extracellular Ca2+. The EF dependent upregulation of [Ca2+]c persisted after EF exposure (P <.05: paired t-test). The results clearly indicate that Ca2+ influx across the plasma membrane is responsible for the enhanced increase of [Ca2+]c in the EF exposed, lectin stimulated cells and that EF has persistent effect on the cells. Although the precise mechanisms of the EF dependent upregulation of [Ca2+]c is not fully elucidated, the present results demonstrate that the 60 Hz EF (6 microA/cm2) affects [Ca2+]c during cell activation via a Ca2+ influx pathway induced by lectin stimulation. | |||||||||||||||
言語 | en | |||||||||||||||
書誌情報 |
en : Bioelectromagnetics 巻 25, 号 3, p. 204-210, 発行日 2004-04 |
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出版者 | ||||||||||||||||
言語 | en | |||||||||||||||
出版者 | Wiley‐Liss | |||||||||||||||
ISSN | ||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||
収録物識別子 | 01978462 | |||||||||||||||
書誌レコードID | ||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||
収録物識別子 | AA00109934 | |||||||||||||||
PubMed番号 | ||||||||||||||||
関連タイプ | isVersionOf | |||||||||||||||
識別子タイプ | PMID | |||||||||||||||
関連識別子 | 15042629 | |||||||||||||||
DOI | ||||||||||||||||
関連タイプ | isVersionOf | |||||||||||||||
識別子タイプ | DOI | |||||||||||||||
関連識別子 | info:doi/10.1002/bem.10187 | |||||||||||||||
フォーマット | ||||||||||||||||
内容記述タイプ | Other | |||||||||||||||
内容記述 | application/pdf | |||||||||||||||
著者版フラグ | ||||||||||||||||
出版タイプ | AM | |||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||
日本十進分類法 | ||||||||||||||||
主題Scheme | NDC | |||||||||||||||
主題 | 427 |