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In Vitro Excystation and Cryoreservation of Ovine and Caprine Sarcocystis species
https://obihiro.repo.nii.ac.jp/records/1727
https://obihiro.repo.nii.ac.jp/records/172770716ded-50aa-462b-a78f-3aca7c2a7a61
名前 / ファイル | ライセンス | アクション |
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Prot.Vol.1-(2).pdf (270.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2006-12-21 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | In Vitro Excystation and Cryoreservation of Ovine and Caprine Sarcocystis species | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cryopreservation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | in vitro excystation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sarcocystis spp. | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | sporozoites | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Horn, Karin
× Horn, Karin× Ono, Kenichiro× Heydorn, Alfred Otto |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as obtained by this method proved to be decontaminated and sporozoites were viable and able to invade Vero cells in tissue culture. Sporozoites were also subjected to cryopreservation in RPMI 1640 containing 10% FCS and 7.5% DMSO. After storage in liquid nitrogen, sporozoites proved to be infective for their intermediate hosts after intraperitoneal injection. | |||||
言語 | en | |||||
書誌情報 |
en : The journal of protozoology research 巻 1, p. 13-21, 発行日 1991-10 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Research Center for Protozoan Molecular Immunology | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 09174427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824856 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Research Center for Protozoan Molecular Immunology | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
言語 | en | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 649 |