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Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
名前 / ファイル | ライセンス | アクション |
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Prot.Vol.16-1,2-2.pdf (202.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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公開日 | 2006-12-20 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | en | |||||||||||||||||
タイトル | Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | eng | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Cryptosporidium parvum | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | p23 | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | ELISA | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | IFAT | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | journal article | |||||||||||||||||
著者 |
坂内, 天
× 坂内, 天× 西川, 義文
WEKO
176
× Seo, Jin-Yong× Nakamura, Chinatsu× Zhang, Sofa× Kimata, Isao× Takashima, Yasuhiro× Li, Junyou× 五十嵐, 郁男
WEKO
58
× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%). Thus, it was demonstrated that the ELISA could be a useful tool for the diagnosis of C. parvum infection in cattle under field conditions to estimate the magnitude of asymptomatic infection in a cattle population and subsequent risk to humans. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
en : The journal of protozoology research 巻 16, 号 1-2, p. 9-15, 発行日 2006-04 |
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出版者 | ||||||||||||||||||
言語 | en | |||||||||||||||||
出版者 | National Research Center for Protozoan Diseases | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 09174427 | |||||||||||||||||
書誌レコードID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AA10824856 | |||||||||||||||||
権利 | ||||||||||||||||||
言語 | en | |||||||||||||||||
権利情報 | National Research Center for Protozoan Diseases | |||||||||||||||||
フォーマット | ||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||
著者版フラグ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
日本十進分類法 | ||||||||||||||||||
主題Scheme | NDC | |||||||||||||||||
主題 | 649 |