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Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis
名前 / ファイル | ライセンス | アクション |
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v16-3,4-1.pdf (98.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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公開日 | 2007-05-24 | |||||||||||||||
タイトル | ||||||||||||||||
言語 | en | |||||||||||||||
タイトル | Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis | |||||||||||||||
言語 | ||||||||||||||||
言語 | eng | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Canine | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | ELISA | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Neospora caninum | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | NcGRA7 | |||||||||||||||
資源タイプ | ||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
資源タイプ | journal article | |||||||||||||||
著者 |
Hara, Olgga A
× Hara, Olgga A× Liao, Min× Baticados, Waren× 坂内, 天× Zhang, Guohong× Zhang, Sofa× Lee, Eung-goo× 西川, 義文
WEKO
176
× Claveria, Florencia G.× Igarashi, Makoto
WEKO
324
× Nagasawa, Hideyuki× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||
内容記述 | Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were positive for antibodies to N. caninum. Of the 32 ELISA-positive samples, 29 (90.62%) were confirmed as positive by Western blot analysis with whole tachyzoite antigens. These results indicate that the recombinant NcGRA7t would be a reliable antigen in ELISA for the diagnosis of canine neosporosis. | |||||||||||||||
言語 | en | |||||||||||||||
書誌情報 |
en : The journal of protozoology research 巻 16, 号 3-4, p. 34-41, 発行日 2006-10 |
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出版者 | ||||||||||||||||
言語 | en | |||||||||||||||
出版者 | National Research Center for Protozoan Diseases | |||||||||||||||
ISSN | ||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||
収録物識別子 | 09174427 | |||||||||||||||
書誌レコードID | ||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||
収録物識別子 | AA10824856 | |||||||||||||||
権利 | ||||||||||||||||
言語 | en | |||||||||||||||
権利情報 | National Research Center for Protozoan Diseases | |||||||||||||||
フォーマット | ||||||||||||||||
内容記述タイプ | Other | |||||||||||||||
内容記述 | application/pdf | |||||||||||||||
著者版フラグ | ||||||||||||||||
出版タイプ | VoR | |||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
日本十進分類法 | ||||||||||||||||
主題Scheme | NDC | |||||||||||||||
主題 | 649 |