WEKO3
アイテム
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The establishment of in vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum
https://obihiro.repo.nii.ac.jp/records/1245
https://obihiro.repo.nii.ac.jp/records/1245647479c6-6938-4f36-8cb8-c7655458d7f0
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S2211320717300027-main.pdf (532.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2017-10-16 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | The establishment of in vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Colorimetric assay | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Drug screening system | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Liquid culture | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Luciferase assay | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Trypanosoma equiperdum | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
菅沼, 啓輔
× 菅沼, 啓輔
WEKO
357
× Yamasaki, Shino× Molefe, Nthatisi Innocentia× Musinguzi, Peter Simon× Davaasuren, Batdorj× Mossaad, Ehab× Narantsatsral, Sandagdorj× Battur, Banzragch× Battsetseg, Badgar× 井上, 昇
WEKO
63
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent and an intracellular dehydrogenase activity-based colorimetric assay using WTS-8 tetrazolium salt (CCK-8 reagent) were used in order to examine the trypanocidal effects of each compound. In addition, the IC50 values of 4 reference trypanocidal compounds (pentamidine, diminazene, suramin and melarsomine) were evaluated and compared using established assays. The IC50 values of these reference compounds corresponded well to previous studies involving other strains of T. equiperdum. The luciferase assay would be suitable for the mass screening of chemical libraries against T.?equiperdum because it allows for the simple and rapid-evaluation of the trypanocidal activities of test compounds, while a simple, inexpensive colorimetric assay will be applicable in developing countries for the evaluation of the drug sensitivity of epidemic trypanosome strains. c 2017 The Authors | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : International Journal for Parasitology: Drugs and Drug Resistance 巻 7, 号 2, p. 200-205, 発行日 2017 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | Elsevier | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 22113207 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
日本十進分類法 | ||||||||||||||
主題Scheme | NDC | |||||||||||||
主題 | 491 |