WEKO3
アイテム
{"_buckets": {"deposit": "b66da4d3-f70b-43a2-8f74-125f4d9088f1"}, "_deposit": {"created_by": 12, "id": "1229", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "1229"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00001229", "sets": ["5"]}, "author_link": ["368", "74", "2606", "2605"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-11-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "C819", "bibliographicPageStart": "C808", "bibliographicVolumeNumber": "311", "bibliographic_titles": [{"bibliographic_title": "American Journal of Physiology-Cell Physiology", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The Cl- secretion via Ca2+-activated Cl- channel (CaCC) is critical for fluid secretion in exocrine glands like the salivary gland. Also in the mammary gland, it has been hypothesized that CaCC plays an important role in the secretion of Cl- and aqueous phase of milk. However, there has been no evidence for the functional expression of CaCC in native mammary secretory (MS) cells of lactating animals. We therefore assessed membrane current in MS cells that were freshly isolated from lactating mice using whole cell patch-clamp techniques. In MS cells, we detected CaCC current that exhibited the following characteristics: 1) Ca2+-dependent activation at the concentrations of sub-micromolar range; 2) voltage-dependent activation; 3) slow kinetics for activation and deactivation; 4) outward rectification of the steady-state current; 5) anion permeability in the sequence of I- \u003e NO3- \u003e Br- \u003e Cl- \u003e\u003e glutamate; 6) inhibition by Cl- channel blockers (niflumic acid, DIDS, and CaCCinh-A01). These characteristics of native CaCC current were similar to reported characteristics of heterologously expressed TMEM16A. RT-PCR analyses showed the expression of multiple CaCC channels including TMEM16A, Best1, and Best3 in the mammary glands of lactating mice. Immuno-histochemical staining revealed the localization of TMEM16A protein at the apical membrane of the MS cells. Collectively, our data strongly suggest that MS cells functionally express CaCC, which is at least partly constituted by TMEM16A. The CaCC such as TMEM16A at the apical membrane of the MS cells may influence the quantity and/ or quality of milk.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Physiological Society", "subitem_publisher_language": "en"}]}, "item_2_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1152/ajpcell.00050.2016", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "American Physiological Society", "subitem_rights_language": "en"}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00521122", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03636143", "subitem_source_identifier_type": "PISSN"}]}, "item_2_subject_21": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "645", "subitem_subject_scheme": "NDC"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "上川昭博(akami@obihiro.ac.jp)"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Submitted by リポジトリ管理者 repo (repo@obihiro.ac.jp) on 2017-11-01T04:41:42Z\nNo. of bitstreams: 1\nKamikawa AJP 2016.pdf: 4951008 bytes, checksum: 1392fc05863d453ca914f42131029f33 (MD5)"}, {"subitem_textarea_value": "Made available in DSpace on 2017-11-01T04:41:42Z (GMT). No. of bitstreams: 1\nKamikawa AJP 2016.pdf: 4951008 bytes, checksum: 1392fc05863d453ca914f42131029f33 (MD5)\n Previous issue date: 2016-11-01"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Kamikawa, Akihiro", "creatorNameLang": "en"}, {"creatorName": "上川, 昭博", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Kamikawa", "familyNameLang": "en"}, {"familyName": "上川", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Akihiro", "givenNameLang": "en"}, {"givenName": "昭博", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "74", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000017180981", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000017180981"}, {"nameIdentifier": "30581665", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=30581665"}, {"nameIdentifier": "read0154445", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0154445"}]}, {"creatorNames": [{"creatorName": "Ichii, Osamu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2605", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakazaki, Junpei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2606", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Toru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "368", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000030211725", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000030211725"}, {"nameIdentifier": "70249960", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70249960"}, {"nameIdentifier": "read0179447", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0179447"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Kamikawa AJP 2016.pdf", "filesize": [{"value": "5.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 5000000.0, "url": {"label": "Kamikawa AJP 2016.pdf", "url": "https://obihiro.repo.nii.ac.jp/record/1229/files/Kamikawa AJP 2016.pdf"}, "version_id": "b3b28056-f47a-4e7a-8da2-dee1ab1d00b6"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "CaCC", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lactation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mammary gland", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mammary secretory cell", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "patch-clamp", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/1229", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-11-01"}, "publish_date": "2017-11-01", "publish_status": "0", "recid": "1229", "relation": {}, "relation_version_is_last": true, "title": ["Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse"], "weko_shared_id": -1}
Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse
https://obihiro.repo.nii.ac.jp/records/1229
https://obihiro.repo.nii.ac.jp/records/1229cb4727a4-ccc0-4761-a8bb-db583576012f
名前 / ファイル | ライセンス | アクション |
---|---|---|
Kamikawa AJP 2016.pdf (5.0 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2017-11-01 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | CaCC | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | lactation | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | mammary gland | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | mammary secretory cell | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | patch-clamp | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
著者 |
上川, 昭博
× 上川, 昭博
WEKO
74
× Ichii, Osamu× Sakazaki, Junpei× Ishikawa, Toru
WEKO
368
|
|||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | The Cl- secretion via Ca2+-activated Cl- channel (CaCC) is critical for fluid secretion in exocrine glands like the salivary gland. Also in the mammary gland, it has been hypothesized that CaCC plays an important role in the secretion of Cl- and aqueous phase of milk. However, there has been no evidence for the functional expression of CaCC in native mammary secretory (MS) cells of lactating animals. We therefore assessed membrane current in MS cells that were freshly isolated from lactating mice using whole cell patch-clamp techniques. In MS cells, we detected CaCC current that exhibited the following characteristics: 1) Ca2+-dependent activation at the concentrations of sub-micromolar range; 2) voltage-dependent activation; 3) slow kinetics for activation and deactivation; 4) outward rectification of the steady-state current; 5) anion permeability in the sequence of I- > NO3- > Br- > Cl- >> glutamate; 6) inhibition by Cl- channel blockers (niflumic acid, DIDS, and CaCCinh-A01). These characteristics of native CaCC current were similar to reported characteristics of heterologously expressed TMEM16A. RT-PCR analyses showed the expression of multiple CaCC channels including TMEM16A, Best1, and Best3 in the mammary glands of lactating mice. Immuno-histochemical staining revealed the localization of TMEM16A protein at the apical membrane of the MS cells. Collectively, our data strongly suggest that MS cells functionally express CaCC, which is at least partly constituted by TMEM16A. The CaCC such as TMEM16A at the apical membrane of the MS cells may influence the quantity and/ or quality of milk. | |||||||||||
言語 | en | |||||||||||
書誌情報 |
en : American Journal of Physiology-Cell Physiology 巻 311, 号 5, p. C808-C819, 発行日 2016-11-01 |
|||||||||||
出版者 | ||||||||||||
言語 | en | |||||||||||
出版者 | American Physiological Society | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 03636143 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA00521122 | |||||||||||
DOI | ||||||||||||
関連タイプ | isVersionOf | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | info:doi/10.1152/ajpcell.00050.2016 | |||||||||||
権利 | ||||||||||||
言語 | en | |||||||||||
権利情報 | American Physiological Society | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | AM | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
日本十進分類法 | ||||||||||||
主題Scheme | NDC | |||||||||||
主題 | 645 |