WEKO3
アイテム
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Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the life-cycle of this parasite.\nMethods: An in vitro kinase assay was performed to assess the phosphorylation activities of a novel CaMK-like protein in T. gondii by using purified proteins with various concentrations of calcium, calmodulin antagonists, or T. gondii glideosome proteins. 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Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog
https://obihiro.repo.nii.ac.jp/records/1212
https://obihiro.repo.nii.ac.jp/records/12123c365a17-e161-4cd6-9839-c25defecaae1
名前 / ファイル | ライセンス | アクション |
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s13071-016-1676-1.pdf (2.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-08-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Calcium calmodulin-dependent protein kinase homolog | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | GAP45 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Phosphorylation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | T. gondii CaMK-related kinase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Toxoplasma gondii | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
加藤, 健太郎
× 加藤, 健太郎× Sugi, Tatsuki× Takemae, Hitoshi× Takano, Ryo× Gong, Haiyan× Ishiwa, Akiko× Horimoto, Taisuke× Akashi, Hiroomi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and a major pathogen of animals and immunocompromised humans, in whom it causes encephalitis. Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the life-cycle of this parasite. Methods: An in vitro kinase assay was performed to assess the phosphorylation activities of a novel CaMK-like protein in T. gondii by using purified proteins with various concentrations of calcium, calmodulin antagonists, or T. gondii glideosome proteins. Indirect immunofluorescence microscopy was performed to detect the localization of this protein kinase by using the antibodies against this protein and organellar maker proteins of T. gondii. Results: We identified a novel CaMK homolog in T. gondii, T. gondii CaMK-related kinase (TgCaMKrk), which exhibits calmodulin-independent autophosphorylation and substrate phosphorylation activity. However, calmodulin antagonists had no effect on its kinase activity. In T. gondii-infected cells, TgCaMKrk localized to the apical ends of extracellular and intracellular tachyzoites. TgCaMKrk phosphorylated TgGAP45 for phosphorylation in vitro. Conclusions: Our data improve our understanding of T. gondii motility and infection, the interaction between parasite protein kinases and glideosomes, and drug targets for protozoan diseases. |
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言語 | en | |||||
書誌情報 |
en : Parasites & Vectors 巻 9, p. 405, 発行日 2016-07-21 |
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出版者 | ||||||
言語 | en | |||||
出版者 | BioMed Central | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 17563305 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1186/s13071-016-1676-1 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 498 |