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Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
名前 / ファイル | ライセンス | アクション |
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s13071-016-1518-1.pdf (3.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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公開日 | 2017-08-16 | |||||||||||||||||||
タイトル | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
タイトル | Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1) | |||||||||||||||||||
言語 | ||||||||||||||||||||
言語 | eng | |||||||||||||||||||
キーワード | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||
主題 | Babesia canis | |||||||||||||||||||
キーワード | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||
主題 | Canine babesiosis | |||||||||||||||||||
キーワード | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||
主題 | BcMSA1 | |||||||||||||||||||
キーワード | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||
主題 | BcSA1 | |||||||||||||||||||
キーワード | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||
主題 | ELISA | |||||||||||||||||||
キーワード | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||
主題 | Immunochromatographic tests | |||||||||||||||||||
資源タイプ | ||||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
資源タイプ | journal article | |||||||||||||||||||
著者 |
Zhou, Mo
× Zhou, Mo× Cao, Shinuo× Luo, Yuzi× Liu, Mingming× Wang, Guanbo× Moumouni, Paul Franck Adjou× Jirapattharasate, Charoonluk× Iguchi, Aiko× Vudriko, Patrick× Terkawi, Mohamad Alaa
WEKO
359
× Lowenstein, Mario× Kern, Angela× 西川, 義文
WEKO
176
× Suzuki, Hiroshi× 五十嵐, 郁男
WEKO
58
× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||||
内容記述 | Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1 and BcSA1 (rBcMSA1 and rBcSA1) expressed in Escherichia coli were purified and injected into mice for production of anti-sera. The native proteins were characterized by Western blot analysis and immunofluorescence. Furthermore, indirect enzyme-linked immunosorbent assays (iELISA) and rapid immunochromatographic tests (ICT) based on rBcMSA1 or rBcSA1 were established and evaluated to test specific antibodies in consecutive plasma samples from two B. canis-infected dogs. Results: Antiserum raised against rBcMSA1 and rBcSA1 recognized the 39 kDa and 44 kDa native proteins by Western blot analysis, respectively. In addition, immunofluorescence and confocal microscopic observations revealed that BcMSA1 was found on the surface of parasites. However, BcSA1 localized in the matrix of the merozoites. The ELISA and ICT based on rBcMSA1 or rBcSA1 could detect specific antibodies in consecutive plasma samples from two B. canis-infected dogs. They showed no cross-reactions against the serum samples collected from dogs experimentally infected with closely related parasites. Conclusion: Taken together, the current results indicated that the rBcMSA1 and rBcSA1 are promising serodiagnostic antigens for developing iELISA and ICT to detect B. canis infection. To our knowledge, this study is the first to report BcMSA1 and BcSA1 as potential antigenic proteins for serodiagnosis of B. canis infection in dogs. |
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言語 | en | |||||||||||||||||||
書誌情報 |
en : Parasites & Vectors 巻 9, p. 257, 発行日 2016-05-03 |
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出版者 | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
出版者 | BioMed Central | |||||||||||||||||||
ISSN | ||||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||||
収録物識別子 | 17563305 | |||||||||||||||||||
DOI | ||||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||||
関連識別子 | info:doi/10.1186/s13071-016-1518-1 | |||||||||||||||||||
権利 | ||||||||||||||||||||
言語 | en | |||||||||||||||||||
権利情報 | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). | |||||||||||||||||||
フォーマット | ||||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||||
著者版フラグ | ||||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
日本十進分類法 | ||||||||||||||||||||
主題Scheme | NDC | |||||||||||||||||||
主題 | 649 |